CAS Registry Number: 475-25-2
CAS Name: 6a,7-Dihydro-3,4,6a,10-tetrahydroxybenz[b]indeno[1,2-d]pyran-9(6H)-one
Additional Names: hydroxybrasilein; hydroxybrazilein
Molecular Formula: C16H12O6
Molecular Weight: 300.26
Percent Composition: C 64.00%, H 4.03%, O 31.97%
Literature References: Not to be confused with hematin. From hematoxylin or logwood extract and NH3 by treatment with air: Engels et al., J. Chem. Soc. 93, 1115 (1908); Rolland, Teintex 3, 261, 322, 460 (1938); Justin-Mueller, Melliand Textilber. 30, 26, 63 (1949), C.A. 46, 8375h (1952).
Properties: Reddish-brown crystals with yellowish-green metallic luster. mp >200°; also stated as 250° with decompn. Sol in about 1700 parts water; slightly sol in alcohol, ether; insol in benzene, chloroform; freely sol in ammonia with brownish-violet color and in dil NaOH with bright red color. Forms salts with heavy metals.
Melting point: mp >200°; also stated as 250° with decompn
Use: As an indicator like hematoxylin; for staining animal tissue, particularly cell nuclei.