Sulfisoxazole Acetyl
309.34Acetamide, N-[(4-aminophenyl)sulfonyl]-N-(3,4-dimethyl-5-isoxazolyl)-.
N-(3,4-Dimethyl-5-isoxazolyl)-N-sulfanilylacetamide
[80-74-0].» Sulfisoxazole Acetyl contains not less than 98.0 percent and not more than 100.5 percent of C13H15N3O4S, calculated on the dried basis.
Packaging and storage—
Preserve in tight, light-resistant containers.
Identification—
B:
Ultraviolet Absorption 
197U
—
197U—
Solution:
10 µg per mL.
Medium:
alcohol.
Absorptivities at 290 nm, calculated on the dried basis, do not differ by more than 3.0%.
Loss on drying 731—
Dry it at 105
for 3 hours: it loses not more than 0.5% of its weight.
731—
Dry it at 105 for 3 hours: it loses not more than 0.5% of its weight.
Ordinary impurities 466—
466—
Test solution:
methanol.
Standard solution:
methanol.
Eluant:
a mixture of toluene and acetone (1:1).
Visualization:
1.
Other requirements—
It meets the requirements for Residue on ignition, Selenium, and Heavy metals under Sulfisoxazole.
Assay—
Transfer about 1 g of Sulfisoxazole Acetyl, accurately weighed, to a 250-mL beaker. Add 15 mL of glacial acetic acid, swirl to dissolve, then add 25 mL of hydrochloric acid and 80 mL of water. Proceed as directed under Nitrite Titration 451, beginning with “Cool to about 15.” Each mL of 0.1 M sodium nitrite is equivalent to 30.94 mg of C13H15N3O4S.
451, beginning with “Cool to about 15.” Each mL of 0.1 M sodium nitrite is equivalent to 30.94 mg of C13H15N3O4S.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
Chromatographic Column—
| Topic/Question | Contact | Expert Committee |
| Monograph | Behnam Davani, Ph.D., M.B.A.
Senior Scientist 1-301-816-8394 |
(MDAA05) Monograph Development-Antivirals and Antimicrobials |
| Reference Standards | Lili Wang, Technical Services Scientist 1-301-816-8129 RSTech@usp.org |
USP32–NF27 Page 3636
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.