Test solution— To a portion of powdered Boluses add 0.1 N hydrochloric acid to obtain a Test solution containing about 4 mg of amoxicillin per mL. Use within 10 minutes after preparation.

Application volume, Developing solvent system, Procedure— Proceed as directed for the Identification test under Amoxicillin Tablets.

Diluent, Mobile phase, Standard preparation, and Chromatographic system— Prepare as directed in the Assay under Amoxicillin.

Assay preparation— Weigh and finely powder not fewer than 5 Boluses. Transfer an accurately weighed portion of the powder, equivalent to about 250 mg of amoxicillin, to a 250-mL volumetric flask, add Diluent to volume, and mix. Sonicate if necessary to ensure complete dissolution of the amoxicillin. Pass a portion of this solution through a filter of 1-µm or finer porosity, and use the filtrate as the Assay preparation. [note—Use this solution within 6 hours.]

Procedure— Proceed as directed for Procedure in the Assay under Amoxicillin. Calculate the quantity, in mg, of amoxicillin (C16H19N3O5S) in the portion of Boluses taken by the formula: in which the terms are as defined therein.