A: Thin-Layer Chromatographic Identification Test 201—

B: The retention times of the relevant analytes in the chromatogram of the Test solution correspond to those in the chromatogram of the Standard solution, as obtained in the test for Content of ginsenosides. The retention time of the peak for ginsenoside Rf in the chromatogram of the Test solution corresponds to that in the chromatogram of the Standard solution, as obtained in the test for Content of ginsenosides.

Solution A, Solution B, Mobile phase, and Chromatographic system— Proceed as directed for Content of ginsenosides under Powdered Asian Ginseng Extract.

Standard solution— Transfer about 200 mg of USP Powdered Asian Ginseng Extract RS, accurately weighed, to a suitable container, dissolve in 5.0 mL of a mixture of water and alcohol (6:4), and filter.

Test solution— Weigh and powder not fewer than 20 Tablets. Transfer an accurately weighed amount of the powder, equivalent to about 200 mg of Powdered Extract, to a conical flask, and extract three times, each with a 20-mL portion of a mixture of methanol and water (8:2), in a 55 bath for 30 minutes, stirring with a magnetic stirrer. Evaporate the combined extracts to dryness in vacuum between 45 and 50. Dissolve the residue in 5.0 mL of a mixture of water and alcohol (6:4), and filter.

Procedure— Proceed as directed for Content of ginsenosides under Powdered Asian Ginseng Extract, except to calculate the quantity, in mg, of each relevant ginsenoside (Rg1, Re, Rb1, Rc, Rb2, and Rd) in the portion of Tablets taken by the formula: in which C is the concentration, in mg per mL, of USP Powdered Asian Ginseng Extract RS in the Standard solution; and the other terms are as defined therein. Calculate the quantity, in mg, of ginsenosides by adding the individual amounts calculated as above.