Fluorouracil Topical Solution
» Fluorouracil Topical Solution contains not less than 90.0 percent and not more than 110.0 percent of the labeled amount of C4H3FN2O2.
Packaging and storage
Preserve in tight containers, and store at controlled room temperature.
Microbial enumeration tests 61 and Tests for specified microorganisms 62
It meets the requirements of the tests for absence of Staphylococcus aureus and Pseudomonas aeruginosa.
Assay
Mobile phase, Standard preparation, and Chromatographic system
Proceed as directed in the Assay under Fluorouracil.
Assay preparation
Transfer an accurately weighed portion of Solution, equivalent to about 20 mg of fluorouracil, to a 100-mL volumetric flask, dilute with water to volume, and mix. Quantitatively dilute a volume of this solution with water to obtain a concentration of 10 µg per mL.
Procedure
Separately inject equal volumes (about 10 µL) of the Standard preparation and the Assay preparation into the chromatograph, record the chromatograms, and measure the peak responses for the major peaks. Calculate the quantity, in mg, of fluorouracil (C4H3FN2O2) in the portion of Solution taken by the formula:
2C(rU / rS)
in which C is the concentration, in µg per mL, of USP Fluorouracil RS in the Standard preparation; and rU and rS are the fluorouracil responses obtained from the Assay preparation and the Standard preparation, respectively.
Auxiliary Information
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Chromatographic Column
USP32NF27 Page 2414
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.
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